unravel.image_io.metadata module#
Use io_metadata
(m
) from UNRAVEL to save x/y and z voxel sizes in microns as well as image dimensions to a metadata file in each sample directory.
- Inputs:
.czi, .nii.gz, .h5, or TIF series (path should be relative to ./sample??)
- Outputs:
./parameters/metadata.txt (path should be relative to ./sample??)
Note
If -d is not provided, the current directory is used to search for sample?? dirs to process.
If the current dir is a sample?? dir, it will be processed.
If -d is provided, the specified dirs and/or dirs containing sample?? dirs will be processed.
If -p is not provided, the default pattern for dirs to process is ‘sample??’.
To test out -d/-p, use the
utils_get_samples
command to see which sample?? dirs will be processed.
- Next command:
reg_prep
for prepping autofluo images for registration
Usage for when metadata is extractable:#
io_metadata -i rel_path/full_res_img [-m parameters/metadata.txt] [-d space-separated list of paths] [-p pattern] [-v]
Usage for when metadata is not extractable:#
io_metadata -i tif_dir -x 3.5232 -z 6 [-m parameters/metadata.txt] [-d space-separated list of paths] [-p pattern] [-v]