unravel.image_io.tif_to_tifs module

Use io_tif_to_tifs (t2t) from UNRAVEL to load a 3D .tif or .ome.tif image and save it as tifs.

Input:

• path/image.tif

Outputs:

• <tif_dir>/slice_0000.tif, <tif_dir>/slice_0001.tif, …

• tif_dir may be specified with -t flag or it will default to <image_name>_tifs

• parameters/metadata.txt (if -m flag is used)

Next command:

io_metadata to specify voxel sizes and image dimensions to parameters/metadata.txt (if metadata is not extractable) reg_prep to prep autofluo images registration

Usage:

io_tif_to_tifs -i <path/image.tif> -t autofl [-v]

unravel.image_io.tif_to_tifs.parse_args()

unravel.image_io.tif_to_tifs.main()